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KMID : 0545120000100020275
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 2 p.275 ~ p.280
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Cloning and Expression of Inulin Fructotransferase Gene of Arthrobacter sp.A-6 in Escharichia coli and Bacillus subtilis
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Kim, Hwa Young
Kim, Chan Wha/Choi, Yong Jin
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Abstract
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The inulin fructotransferase (depolymerizing) (IFTase, EC 2.4.1.93) gene of Arthrobacter sp. A-6 was cloned and expressed in Escherichia coli and Bacillus subtilis. The IFTase gene consisted of an ORF of 1,311 nucleotides encoding a polypeptide of 436 amino acids containing a signal peptide of 31 amino acids in the N-terminus. The molecular mass of the IFTase based on the nucleotide sequence was calculated to be 46,116 Da. The recombinant E. coli DH5α cells expressing the Arthmbacter sp. A-6 IFTase gene produced most of the IFTase intracellularly. In contrast, the recombinant B. subtilis DB 104 carrying the IFTase gene on a B. subtilis-E. coli expression vector secreted the IFTase into the culture fluid efficiently.
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